The 80,000g supernatant of larval midgut homogenates of the tobacco hornworm, Manduca sexta, was fractionated by affinity chromatography on Blue Sepharose CLdB and by anion exchange chromatography on Q Sepharose. Both methods resolved one major 3-oxoecdysteroid 3a-reductase and three major 3-oxoecdy
โฆ LIBER โฆ
Ecdysone oxidase and 3-oxoecdysteroid reductases in Manduca sexta midgut: Kinetic parameters
โ Scribed by Gunter F. Weirich; Malcolm J. Thompson; James A. Svoboda
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 956 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0739-4462
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The larval midgut of the tobacco hornworm, Manduca sexta, has high ecdysone 20-monooxygenase (EZOMO) activity, located both in the mitochondria and in the microsomes. The apparent kinetic parameters for E20MO in mitochondria and microsomes were determined. The K , s (for ecdysone) of the rnitochondr