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Ecdysone oxidase and 3-oxoecdysteroid reductases in Manduca sexta: Developmental changes and tissue distribution

✍ Scribed by Gunter F. Weirich; Mark F. Feldlaufer; James A. Svoboda

Publisher: John Wiley and Sons
Year: 1993
Tongue: English
Weight: 773 KB
Volume: 23
Category: Article
ISSN: 0739-4462
DOI: 10.1002/arch.940230406

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The 80,000g supernatant of larval midgut homogenates of the tobacco hornworm, Manduca sexta, was fractionated by affinity chromatography on Blue Sepharose CLdB and by anion exchange chromatography on Q Sepharose. Both methods resolved one major 3-oxoecdysteroid 3a-reductase and three major 3-oxoecdy