Dynamics of paramagnetic agents by off-resonance rotating frame technique
β Scribed by Huiming Zhang; Yang Xie
- Book ID
- 108184796
- Publisher
- Elsevier Science
- Year
- 2006
- Tongue
- English
- Weight
- 561 KB
- Volume
- 183
- Category
- Article
- ISSN
- 1090-7807
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15N off-resonance rotating frame relaxation can be applied to the study of internal dynamics in proteins in the millisecond to microsecond regime. We show that the performance of existing methods can be improved by application of simultaneous amplitude and phase-modulated adiabatic RF pulses to alig
The '"C off-resonance rotating frame spin-lattice relaxation technique is applicable to the study of protein rotational diffusion behavior in both model in vitro and in vivo systems. The original formalism of James and co-workers [(1978) J. Am. Chem. SOC. 100, 3590-35941 was constrained by the assum