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Dynamic profiling of amino acid transport and metabolism in Chinese hamster ovary cell culture

โœ Scribed by Kyriakopoulos, Sarantos; Polizzi, Karen M; Kontoravdi, Cleo


Book ID
121591557
Publisher
BioMed Central
Year
2013
Tongue
English
Weight
344 KB
Volume
7
Category
Article
ISSN
1753-6561

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## Abstract To determine if Chinese Hamster Ovary (CHO) cells in culture are able to metabolize steroids, CHO cells were incubated in defined medium with [^14^C]pregnenolone. As shown, [^14^C]pregnenolone is metabolized to progesterone and other ฮด^5^3ฮฒ steroids; this steroidogenic response is appre