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Dynamic changes in p27kip1 variant expression in activated lymphocytes

✍ Scribed by B. Yaroslavskiy; S.C. Watkins; Sean Alber; Richard A. Steinman

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
317 KB
Volume
83
Category
Article
ISSN
0730-2312

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✦ Synopsis

Abstract

The p27Kip1 cell cycle inhibitor (p27) has emerged as a critical mediator of normal cellular growth control. We report the expression of a 24 kD C‐terminal variant of p27 in normal peripheral blood lymphocytes. This variant is rapidly degraded in a proteasome‐dependent manner when lymphocytes are activated by interleukin‐2 or by superantigen. Whereas p24 degradation is complete within 16 h of mitogen addition, full‐length p27 is decreased only modestly over 72 h of mitogen exposure and is present in activated and cycling lymphocytes. Persistent p27 is present in a complex with cyclin D3 in activated lymphocytes, and is localized both in the nucleus and cytoplasm. These results indicate that lymphocytes exiting from quiescence use several mechanisms to overcome the p27Kip1‐enforced cell cycle checkpoint, and that elimination of p27 is not required for cell cycle entry. J. Cell. Biochem. 83: 380–389, 2001. © 2001 Wiley‐Liss, Inc.

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