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Duplexes of 21-nucleotide RNAs mediate RNA interference in differentiated mouse ES cells

โœ Scribed by Gang-Ming Zou; Wei Wu; Jianjun Chen; Janet D. Rowley


Book ID
114191897
Publisher
Portland Press
Year
2003
Tongue
English
Weight
290 KB
Volume
95
Category
Article
ISSN
0248-4900

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## Abstract Summary: We examined whether suppression of Oct4 via RNA interference (RNAi) would affect embryonic stem (ES) cell lineage choice. Cells were transfected with plasmids containing an independently expressed reporter gene and an RNA polymerase type III promoter to constitutively express s