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Differentiation of Mouse Embryonic Stem Cells after RNA Interference-Mediated Silencing of OCT4 and Nanog

✍ Scribed by Shelley R. Hough; Ian Clements; Peter J. Welch; Kristin A. Wiederholt

Book ID
111845528
Publisher
AlphaMed Press
Year
2006
Tongue
English
Weight
661 KB
Volume
24
Category
Article
ISSN
1066-5099

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## Abstract Summary: We examined whether suppression of Oct4 via RNA interference (RNAi) would affect embryonic stem (ES) cell lineage choice. Cells were transfected with plasmids containing an independently expressed reporter gene and an RNA polymerase type III promoter to constitutively express s