<p>This volume provides current up-to-date protocols for preparing the ovary for various imaging techniques, genetic protocols for generating mutant clones, mosaic analysis and assessing cell death. Chapters address methods for performing genome wide gene expression analysis and bioinformatics for s
Drosophila Oogenesis: Methods and Protocols
โ Scribed by Michelle S. Giedt; Tina L. Tootle
- Publisher
- Springer Nature
- Year
- 2023
- Tongue
- English
- Leaves
- 447
- Category
- Library
No coin nor oath required. For personal study only.
โฆ Synopsis
This detailed volume compiles numerous methods to explore fly oogenesis. Beginning with updated protocols from isolating and staining the ovary to numerous imaging techniques and genetic protocols for cell-specific assessment and CRISPR-mediated mutagenesis, the volume continues by detailing techniques from assessing cytoskeletal structures to uncovering protein-protein interactions, with closing chapters exploring how Drosophila oogenesis can be used in the classroom and in outreach programs to increase interest in biomedical research, STEM education, and STEM careers. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Drosophila Oogenesis: Methods and Protocols serves as an ideal guide for both new and expert researchers working with this robust and versatile model organism.
๐ SIMILAR VOLUMES
<p><p>This volume provides current up-to-date protocols for preparing the ovary for various imaging techniques, genetic protocols for generating mutant clones, mosaic analysis and assessing cell death. Chapters address methods for performing genome wide gene expression analysis and bioinformatics fo
<span>This detailed volume compiles numerous methods to explore fly oogenesis. Beginning with updated protocols from isolating and staining the ovary to numerous imaging techniques and genetic protocols for cell-specific assessment and CRISPR-mediated mutagenesis, the volume continues by detailing t
<p><P>With its long-standing tradition as a model organism, many techniques using <EM>Drosophila melanogaster</EM> have been established and continue to be developed, while the recent invention of techniques allowing the knock-down of genes by RNA interference and gene replacement by homologous reco
This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and