This detailed volume compiles numerous methods to explore fly oogenesis. Beginning with updated protocols from isolating and staining the ovary to numerous imaging techniques and genetic protocols for cell-specific assessment and CRISPR-mediated mutagenesis, the volume continues by detailing techniq
Drosophila Oogenesis: Methods and Protocols
โ Scribed by Diana P. Bratu, Gerard P. McNeil
- Publisher
- Humana Press
- Year
- 2015
- Tongue
- English
- Leaves
- 259
- Series
- Methods in Molecular Biology 1328
- Category
- Library
No coin nor oath required. For personal study only.
โฆ Synopsis
This volume provides current up-to-date protocols for preparing the ovary for various imaging techniques, genetic protocols for generating mutant clones, mosaic analysis and assessing cell death. Chapters address methods for performing genome wide gene expression analysis and bioinformatics for studies of RNA-protein interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Drosophila Oogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field.
โฆ Table of Contents
Front Matter....Pages i-xi
Back Matter....Pages 1-20
....Pages 21-28
โฆ Subjects
Cell Biology; Animal Models
๐ SIMILAR VOLUMES
<p><p>This volume provides current up-to-date protocols for preparing the ovary for various imaging techniques, genetic protocols for generating mutant clones, mosaic analysis and assessing cell death. Chapters address methods for performing genome wide gene expression analysis and bioinformatics fo
<span>This detailed volume compiles numerous methods to explore fly oogenesis. Beginning with updated protocols from isolating and staining the ovary to numerous imaging techniques and genetic protocols for cell-specific assessment and CRISPR-mediated mutagenesis, the volume continues by detailing t
<p><P>With its long-standing tradition as a model organism, many techniques using <EM>Drosophila melanogaster</EM> have been established and continue to be developed, while the recent invention of techniques allowing the knock-down of genes by RNA interference and gene replacement by homologous reco
This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and