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Drosophila alcohol dehydrogenase: A novel reporter gene for use in mammalian embryos

✍ Scribed by Nielsen, Loretta L. ;Pedersen, Roger A.

Publisher: John Wiley and Sons
Year: 1991
Tongue: English
Weight: 757 KB
Volume: 257
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1402570117

No coin nor oath required. For personal study only.

✦ Synopsis

A reporter gene construct containing the Drosophila alcohol dehydrogenase (ADH) gene under regulation of the Rous sarcoma virus long terminal repeat (RSV LTR) was microinjected into mouse zygote pronuclei. ADH activity after injection of the RSV-ADH construct was visualized in cultured embryos by means of a simple histochemical enzyme assay. The RSV LTR was an efficient promoter that led to abundant ADH activity at all stages of preimplantation development. There was a high incidence of mosaicism among stained embryos.

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