A reporter gene construct containing the Drosophila alcohol dehydrogenase (ADH) gene under regulation of the Rous sarcoma virus long terminal repeat (RSV LTR) was microinjected into mouse zygote pronuclei. ADH activity after injection of the RSV-ADH construct was visualized in cultured embryos by me
β¦ LIBER β¦
A novel triple fusion reporter system for use in gene trap mutagenesis
β Scribed by Anestis Tsakiridis; Elena Tzouanacou; Osmany Larralde; Tim M. Watts; Valerie Wilson; Lesley Forrester; Joshua M. Brickman
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 401 KB
- Volume
- 45
- Category
- Article
- ISSN
- 1526-954X
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Recent demonstrations of loss of heterozygosity in a wide variety o f human cancers suggest that large multilocus genetic deletions (presumably including tumor suppressor genes) constitute a major class of genetic alteration in human carcinogenesis. Here we show that a bifunctional fusion gene (Hytk