Down-regulation of Telomerase Activity Is an Early Event in the Differentiation of HL60 Cells
โ Scribed by Ericka Savoysky; Kenji Yoshida; Toshihiko Ohtomo; Yohsuke Yamaguchi; Ken-ichi Akamatsu; Tatsumi Yamazaki; Shonen Yoshida; Masayuki Tsuchiya
- Book ID
- 115579025
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 157 KB
- Volume
- 226
- Category
- Article
- ISSN
- 0006-291X
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The steady-state level of nucleophosmin/B23 mRNA decreased during berberine-induced (25 microg/ml, 24 to 96 hr) apoptosis of human leukemia HL-60 cells. A decline in telomerase activity was also observed in HL-60 cells treated with berberine. A stable clone of nucleophosmin/B23 overexpressed in HL-6
Telomerase activity provides a mechanism for the unlimited division potential of neoplastic cells. Induced differentiation of these cells was found to be associated with repression of telomerase activity irrespective of the inducing agent. We have employed a series of sublines of human promyelocytic