DNA/RNA synthesis and labelling

✍ Scribed by Michael J. Gait

• Publisher: Elsevier Science
• Year: 1991
• Tongue: English
• Weight: 904 KB
• Volume: 2
• Category: Article
• ISSN: 0958-1669
• DOI: 10.1016/0958-1669(91)90062-a

✦ Synopsis

Reliable methods of machine-aided RNA synthesis have been established to complement those for DNA assembly. Oligonucleotides containing thio-modified backbones and 2'-O-alkyl sugars head the list of many newly available analogues. Biotin, fluorescent agents and many reporter groups can be conveniently introduced into oligonucleotides in multiples by phosphoramidite or H-phosphonate chemistry.