DNA'Protein Interactions: Principles and Protocols

✍ Scribed by Tom Moss

Publisher: Humana Press
Year: 2001
Tongue: English
Leaves: 636
Series: Methods in Molecular Biology
Edition: 2nd
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions and define their components, their manner of interaction, and their manner of function, both in the cell and in the test tube. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to understand DNA-protein interactions.

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