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DNA transfection of Escherichia coli by electroporation

โœ Scribed by Akira Taketo


Book ID
119119742
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
517 KB
Volume
949
Category
Article
ISSN
0167-4781

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Infectivity of Mu DNA was demonstrated in Ca+ +-treated Escherichia coli cells that lacked the nucleases Exo V and Endo I. The efficiency of transfection is about 10(-7) per phage equivalent. Infectivity is destroyed by denaturation of Mu DNA, and cannot be restored by renaturation.

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