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DNA Sequencing with a Hexamer String Primer and Dye-Labeled Terminators

✍ Scribed by W.Y. Hou; L.M. Smith

Publisher: Elsevier Science
Year: 1994
Tongue: English
Weight: 628 KB
Volume: 221
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1994.1389

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✦ Synopsis

A method for fluorescence-based DNA sequencing using a hexamer string primer and dye-labeled terminators is described. Sequencing protocols have been determined that provide an average read length of 393 bases with 99.6% base-calling accuracy for single-stranded M13mp18 DNA, and an average read length of 367 bases with 99.4% base-calling accuracy for double-stranded M13mp19 DNA. The signal intensity is stronger than that obtained with a standard 18-mer primer, while the resolution and length of readable sequence are comparable.

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