DNA Sequencing: Optimizing Process and Analysis
β Scribed by Mostafa Ronaghi; Arjang Hassibi
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 91 KB
- Volume
- 17
- Category
- Article
- ISSN
- 1042-0533
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π SIMILAR VOLUMES
The frequency, position, and type of errors were analyzed for sequence data which were generated with the help of (I) a manual digitizer, (II) an off-line automated film reader, and (III) an on-line automated gel reader. About 400 film/gel readings were analyzed and the results were used to generate
## Abstract We show that the fine oscillatory structure of the DNA melting curve can be used to determine explicitly the nucleotide composition and the order of certain domains within the DNA. If DNA is specifically fragmented, the order of fragments can be learned directly from a comparison of the