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Direct DNA Sequencing Following SSCP Analysis

โœ Scribed by I. Petersen; H. Ohgaki; B. Ludeke; P. Kleihues

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
193 KB
Volume
218
Category
Article
ISSN
0003-2697

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Sensitivity of single-strand conformation polymorphism analysis of PCR products (PCR-SSCP analysis) is known to be decreased when the DNA fragments are longer than 300 bp. We examined effects of buffer ions in an attempt to extend the length limit of the analysis. It has been noted that addition of