DNA Sequencing: Clinical Applications of New DNA Sequencing Technologies
โ Scribed by Dewey, F. E.; Pan, S.; Wheeler, M. T.; Quake, S. R.; Ashley, E. A.
- Book ID
- 111895520
- Publisher
- Lippincott Williams and Wilkins
- Year
- 2012
- Tongue
- English
- Weight
- 567 KB
- Volume
- 125
- Category
- Article
- ISSN
- 0009-7322
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An entirely new method of sequencing DNA has been devised that does not use electrophoresis, radioactivity, or fluorescence. The method works by measuring pyrophosphate generated by the DNA polymerization reaction. DNA and DNA polymerase are held by a DEAE-Sepharose column and solutions containing d
Since the independent invention of DNA sequencing by Sanger and by Gilbert 30 years ago, it has grown from a small scale technique capable of reading several kilobase-pair of sequence per day into today's multibillion dollar industry. This growth has spurred the development of new sequencing technol