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A new method of sequencing DNA

✍ Scribed by Edward David Hyman

Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
953 KB
Volume
174
Category
Article
ISSN
0003-2697

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✦ Synopsis

An entirely new method of sequencing DNA has been devised that does not use electrophoresis, radioactivity, or fluorescence. The method works by measuring pyrophosphate generated by the DNA polymerization reaction. DNA and DNA polymerase are held by a DEAE-Sepharose column and solutions containing different dNTPs are pumped through. The pyrophosphate generated is measured continuously by a device consisting of a series of columns containing enzymes covalently attached to Sepharose. The alternating copolymer poly(dA.dT) is sequenced as an illustration of the method. Future improvements that will facilitate automation are discussed.

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