A new method for the determination of the relative affinity of a ligand against various DNA sequences by electrospray ionization mass spectrometry. Application to a polyamide minor groove binder

✍ Scribed by William Buchmann; Alexandre Boutorine; Ludovic Halby; Jeanine Tortajada; Edwin De Pauw

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 437 KB
• Volume: 44
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/jms.1592

✦ Synopsis

Abstract

A new method for the determination of the relative affinity of a ligand against various dsDNA sequences is presented by using electrospray ionization time‐of‐flight mass spectrometry (ESI‐QTOF) mass spectrometry. The principle is described here through the complexation of double‐stranded DNA by a polyamide ligand including twelve N‐methylpyrrole rings. However this method could be applied to other ligands especially when dissociation constants (K~d~) are in nanomolar range. This method does not require knowing the ligand concentration accurately. It allows determination of the relative affinity of a ligand against various dsDNA sequences for 1 : 1 complex stoichiometries in a quick manner without labeling. Copyright © 2009 John Wiley & Sons, Ltd.