## Abstract We show that the fine oscillatory structure of the DNA melting curve can be used to determine explicitly the nucleotide composition and the order of certain domains within the DNA. If DNA is specifically fragmented, the order of fragments can be learned directly from a comparison of the
DNA sequencing and helix–coil transition. IV. Very long DNAs
✍ Scribed by M. Ya. Azbel
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1980
- Tongue
- English
- Weight
- 143 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0006-3525
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Helix–coil transition in very long DNA (containing hundreds of thousands of nucleotides or more) is considered. Certain statistical information about DNA nucleotide sequence is obtained.
📜 SIMILAR VOLUMES
## Abstract An explicit analytical theory of DNA melting is constructed. It accounts for the loop entropy and the elasticity of DNA strands. Explicit analytical formulas are presented for the melting curves of natural DNA and periodic polymers. The nature of the DNA helix–coil transition is investi
Protamine-DNA complexes prepared by the method of direct and slow mixing in 2.5 x lO-4M EDTA, pH 8.0, have been studied by thermal denaturation and circular dichroism. The complexes show biphasic melting with T, at about 50°C corresponding to the melting of free DNA regions and T,' at about 92°C cor
On p. 1713 in the first line of the Synopsis the value for [8]242.5 should be -2.2 X deg deg cm2/dmole. Also, on p. 1716, fourth paragraph, line cm2/dmole instead of -2 X 3, the DNA is a solution of A = -0.5 units, not 0.05 units.
## Abstract A strong, positive, extrinsic CD band ([θ]~242.5~ = ∼2 × 10^−3^ deg cm^2^/dmole) has been observed for a α‐bromo‐poly[methylene‐1,4‐phenylenecarbonyloxyethylene(dimethylamino) bromide] (I). The extrinsic Cotton effect is attributed to the ordered arrangement of the aromatic chromophores
## Abstract Thermal denaturation and renaturation of directly mixed and reconstituted polylysine–DNA, directly mixed polylysine–nucleohistone complexes, and NaCl‐treated nucleohistones in 2.5 × 10^−4^ __M__ EDTA, pH 8.0 have been studied. At the same input ratio of polylysine to DNA, the percent of