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DNA sequencing and helix–coil transition. II. Loop entropy and DNA melting

✍ Scribed by M. Ya. Azbel

Publisher
Wiley (John Wiley & Sons)
Year
1980
Tongue
English
Weight
664 KB
Volume
19
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

An explicit analytical theory of DNA melting is constructed. It accounts for the loop entropy and the elasticity of DNA strands. Explicit analytical formulas are presented for the melting curves of natural DNA and periodic polymers. The nature of the DNA helix–coil transition is investigated, and it is found to crucially depend on the nucleotide sequence.

📜 SIMILAR VOLUMES

DNA sequencing and helix–coil transition
DNA sequencing and helix–coil transition. III. DNA sequencing
✍ M. Ya. Azbel 📂 Article 📅 1980 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 877 KB

## Abstract We show that the fine oscillatory structure of the DNA melting curve can be used to determine explicitly the nucleotide composition and the order of certain domains within the DNA. If DNA is specifically fragmented, the order of fragments can be learned directly from a comparison of the

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## Abstract A strong, positive, extrinsic CD band ([θ]~242.5~ = ∼2 × 10^−3^ deg cm^2^/dmole) has been observed for a α‐bromo‐poly[methylene‐1,4‐phenylenecarbonyloxyethylene(dimethylamino) bromide] (I). The extrinsic Cotton effect is attributed to the ordered arrangement of the aromatic chromophores

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## Abstract Thermal denaturation and renaturation of directly mixed and reconstituted polylysine–DNA, directly mixed polylysine–nucleohistone complexes, and NaCl‐treated nucleohistones in 2.5 × 10^−4^ __M__ EDTA, pH 8.0 have been studied. At the same input ratio of polylysine to DNA, the percent of