๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

DNA sequence determinants of CAP-induced bending and protein binding affinity

โœ Scribed by Gartenberg, Marc R.; Crothers, Donald M.

Book ID
109754687
Publisher
Nature Publishing Group
Year
1988
Tongue
English
Weight
907 KB
Volume
333
Category
Article
ISSN
0028-0836

No coin nor oath required. For personal study only.

๐Ÿ“œ SIMILAR VOLUMES

Selection of High-Affinity Binding Sites
Selection of High-Affinity Binding Sites for Sequence-Specific, DNA Binding Proteins from Random Sequence Oligonucleotides
โœ S. Pierrou; S. Enerback; P. Carlsson ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 695 KB

We describe a rapid and sensitive method to isolate sets of high-affinity binding sites for sequence-specific DNA binding proteins. The DNA binding domain of the protein is expressed in Escherichia coli as a fusion with glutathione S-transferase (GST). Binding reactions are set up with total soluble

Affinity chromatography of a sequence-sp
Affinity chromatography of a sequence-specific DNA binding protein using teflon-linked oligonucleotides
โœ Craig H. Duncan; Sharon L. Cavalier ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 594 KB

An affinity matrix was constructed by synthesis of a DNA oligonucleotide on a Teflon fiber support followed by deblocking and hybridization of the complementary strand. It was used to purify a sequence-specific binding protein at least 100-fold to near homogeneity. This matrix is easily fabricated o