✦ LIBER ✦

DNA replication and nuclear architecture

✍ Scribed by Françoise Jaunin; Stanislav Fakan

Publisher: John Wiley and Sons
Year: 2002
Tongue: English
Weight: 249 KB
Volume: 85
Category: Article
ISSN: 0730-2312
DOI: 10.1002/jcb.10115

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The model of in situ DNA replication provided by immunofluorescence and confocal imaging is compared with observations obtained by electron microscopic studies. Discrepancies between both types of observations call into question the replication focus as a persistent nuclear structure and as a replication entity where DNA replication takes place. Most electron microscopic analyses reveal that replication sites are confined to dispersed chromatin areas at the periphery of condensed chromatin, and the distribution of replication factors exhibits the same localization pattern. Moreover, rapid migration of newly synthesized DNA from the replication sites towards the interior of condensed chromatin regions obviously takes place during S‐phase. It implies modifications of replication domains, hardly detectable by fluorescence microscopy. The confrontation of different observations carried out at light microscopic or electron microscopic levels of resolution lead to a conclusion that a combination of in vivo fluorescence analysis with a subsequent ultrastructural investigation performed on the same cells will represent an optimal approach in future studies of nuclear functions in situ. J. Cell. Biochem. 85: 1–9, 2002. © 2002 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Nuclear matrix support of DNA replicatio

Nuclear matrix support of DNA replication

✍ Boyka Anachkova; V. Djeliova; G. Russev 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 190 KB

## Abstract In higher eukaryotic cells, DNA is tandemly arranged into 10^4^ replicons that are replicated once per cell cycle during the S phase. To achieve this, DNA is organized into loops attached to the nuclear matrix. Each loop represents one individual replicon with the origin of replication

Nuclear domain 10, the site of DNA virus

Nuclear domain 10, the site of DNA virus transcription and replication

✍ Gerd G. Maul 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 154 KB 👁 2 views

Within the highly organized nuclear structure, specific nuclear domains (ND10) are defined by accumulations of proteins that can be interferon-upregulated, implicating ND10 as sites of a nuclear defense mechanism. Compatible with such a mechanism is the deposition of herpesvirus, adenovirus, and pa

The sperm nuclear matrix is required for

The sperm nuclear matrix is required for paternal DNA replication

✍ Jeffrey A. Shaman; Yasuhiro Yamauchi; W. Steven Ward 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 231 KB

## Abstract The mammalian sperm nucleus provides an excellent model for studying the relationship between the formation of nuclear structure and the initiation of DNA replication. We previously demonstrated that mammalian sperm nuclei contain a nuclear matrix that organizes the DNA into loop domain

DNA interactions with the nuclear matrix

DNA interactions with the nuclear matrix and spatial organization of replication and transcription

✍ S. V. Razin 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 671 KB

Proteolytic modification of human complement protein C9: loss of poly (C9) and circular lesion formation without impairment of function. Proc. Nat. Acad. Sci. USA 19 YAMAIZUMI, M., UCHIDA, T. & OKADA, Y. (1979). Macromolecules can penetrate the host cell membrane during the early period of incubatio

Concepts in nuclear architecture

✍ Tom Misteli 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 199 KB

## Abstract Genomes are defined by their primary sequence. The functional properties of genomes, however, are determined by far more complex mechanisms and depend on multiple layers of regulatory control processes. A key emerging contributor to genome function is the architectural organization of t

Connecting nuclear architecture and geno

Connecting nuclear architecture and genomic function

✍ Ronald Berezney; Michael Mortillaro; Hong Ma; Chunling Meng; Jagath Samarabandu; 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 351 KB 👁 2 views

Research in our laboratory employs a dual approach to correlating genomic function and regulation with nuclear architecture. In one series of projects we are identifying, cloning, and studying the molecular, genetic, and functional properties of the nuclear matrix proteins which comprise the three-d