DNA microdensitometry as a measure of cycling-non-cycling activity in aged human diploid cells in culture
β Scribed by G.L. Grove; R.B. Mitchell
- Book ID
- 119024749
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 520 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0047-6374
No coin nor oath required. For personal study only.
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## Abstract Using a combination of DNAβcytophotometry and tritiated thymidineβautoradiography, we have shown that the majority of nondividing cells in serially propagated human diploid cell populations have the 2C DNA content consistent with their being arrested in the G~1~ phase of the diploid cel
There are few data available on cell cycle events that occur when proliferation of normal cells in culture is curtailed due to "natural aging" of the culture conditions. Stathmokinetic and cytofluorometry studies were performed on PHA-stimulated human lymphocyte cultures for eight consecutive days.