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DNA synthesis and proliferation of human lymphocytes in vitro: III. Fate of cycling cells in aging cultures of phytohemagglutinin stimulated human lymphocytes

✍ Scribed by John Mendelsohn; Dorothy Hudig; Janice Castagnola

Publisher: John Wiley and Sons
Year: 1981
Tongue: English
Weight: 813 KB
Volume: 106
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041060103

No coin nor oath required. For personal study only.

✦ Synopsis

There are few data available on cell cycle events that occur when proliferation of normal cells in culture is curtailed due to "natural aging" of the culture conditions. Stathmokinetic and cytofluorometry studies were performed on PHA-stimulated human lymphocyte cultures for eight consecutive days. Cell proliferation peaked on day 5 and then gradually decreased. Percent labeled mitosis curves performed each day demonstrated that, for those cells which progressed to mitosis, the cell cycle time remained constant at 18 & 1 hour throughout the entire period of culture. However when the fate of all cells pulse-labeled with "-thymi-

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