## Abstract The components of unidirectional K influx and efflux have been investigated in the 3T3 cell and the SV40 transformed 3T3 cell in exponential and stationary growth phase. Over the cell densities used for transport experiments the 3T3 cell goes from exponential growth to density dependent
DNA methylation levels in normal and chemically-transformed mouse 3T3 cells
โ Scribed by Edward S. Diala; Robert M. Hoffman
- Book ID
- 118316261
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 324 KB
- Volume
- 104
- Category
- Article
- ISSN
- 0006-291X
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๐ SIMILAR VOLUMES
3T3 and SV-40 transformed 3T3 mouse fibroblasts were cultured i n media with serum and antibiotics plus ammonia (NH3 z NH,') added as NHaC1. Both cell lines cultured without added ammonia showed normal morphology and multiplication even though ammonia in the medium at the end of the culture period r
Total cellular calcium levels do not change when 3T3-4a cells stop proliferating due to serum depletion, or when serum-arrested quiescent cells are incubated for up to 44 hours in calcium-deficient medium (-10 pM Ca++). Upon stimulation with dialyzed serum cells enter S and progress through