DNA: DNA Hybridization studies in black flies
โ Scribed by U-Ik Sohn; K. H. Rothfels; N. A. Straus
- Publisher
- Springer
- Year
- 1975
- Tongue
- English
- Weight
- 491 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0022-2844
No coin nor oath required. For personal study only.
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Sheila J. Wood ERDEC Research Directorate, SCBRD-RTE, Aberdeen Proving Ground, Maryland 21010 Received February 13, 1992; accepted May 12, 1992 Hybridization between complementary DNA oligonucleotides was performed in the real time monitoring flow analysis system, BIAcore. Avidin was covalently imm
A fast and simple procedure for the hybridization of radioactive probes directly to DNA in agarose gels is described. Restriction endonuclease-digested DNA is fractionated by electrophoresis in agarose gel. After drying, the DNA in the gel is denatured with alkali and annealed with a radioactive pro