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DNA dideoxy sequencing with T7 DNA polymerase: improved sequencing data by the addition of manganese chloride

✍ Scribed by Tom Kristensen; Hartmut Voss; Wilhelm Ansorge; Hans Prydz


Book ID
113288949
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
306 KB
Volume
6
Category
Article
ISSN
0168-9525

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The dideoxy chain termination method using deoxy-7-deazaguanosine triphosphate (dc 7 GTP)in place of dGTP was found to be very useful. Sequencing of a part of the human N-myc gene having 85% GC content is impossible by the original method using dGTP, because of compression of bands. However, the nuc