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Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP

✍ Scribed by Mizusawa, Saeko (author);Nishimura, Susumu (author);Seela, Frank (author)


Book ID
115465417
Publisher
Oxford University Press
Year
1986
Tongue
English
Weight
823 KB
Volume
14
Category
Article
ISSN
0305-1048

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Improvement of the dideoxy chain termina
✍ Mizusawa, Saeko (author);Nishimura, Susumu (author);Seela, Frank (author) πŸ“‚ Article πŸ“… 1986 πŸ› Oxford University Press 🌐 English βš– 823 KB

The dideoxy chain termination method using deoxy-7-deazaguanosine triphosphate (dc 7 GTP)in place of dGTP was found to be very useful. Sequencing of a part of the human N-myc gene having 85% GC content is impossible by the original method using dGTP, because of compression of bands. However, the nuc