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DNA damage production in CHO cells at elevated temperatures

✍ Scribed by Raymond L. Warters; Lucy M. Brizgys; Joann Axtell-Bartlett

Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
735 KB
Volume
124
Category
Article
ISSN
0021-9541

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✦ Synopsis

Induction of DNA lesions in the nucleus of Chinese hamster ovary (CHO) cells was observed at hypertherrnic temperatures using the alkaline filter elution and the alkaline sucrose gradient sedimentation methods. These lesions were observed principally at temperatures greater than 45OC with an activation energy of 140 kcallmole. On alkaline sucrose gradients the cell genome was reduced to a 140 S or 2 x lo8 dalton subunit of DNA independent of increasing exposure time at temperatures above 45OC. The large thermal activation energy and the limited DNA size reduction suggest the possible involvement of thermal denaturation of a nuclear polypeptide in the production of these nuclear lesions.

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