Formaldehyde production by Tris buffer in peptide formulations at elevated temperature

✍ Scribed by Yuan Song; Richard L. Schowen; Ronald T. Borchardt; Elizabeth M. Topp

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 135 KB
• Volume: 90
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.1073

✦ Synopsis

This technical note provides evidence for the degradation of Tris buffer in a peptide formulation stored at elevated temperature (70 C). The buffer degrades to liberate formaldehyde, which is shown to react with the peptide tyrosine residue. Those involved in peptide/protein formulation should be aware of the possible instability in this common biological buffer.