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DNA content and chromosome number of a heteroploid human tumour cell line

✍ Scribed by F. T. Bosman; F. C. A. Groen; M. Ploeg

Publisher: Springer
Year: 1979
Tongue: English
Weight: 667 KB
Volume: 60
Category: Article
ISSN: 1432-119X
DOI: 10.1007/bf00495753

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✦ Synopsis

In this paper investigations concerning the relation between variability of chromosome number and variability of DNA content within the cells of a tumour stemline are reported. A highly heteroploid human tumour cell line was used, which was derived from a chondrosarcoma. Flow cytometrical and scanning cytophotometrical measurements confirmed the heteroploid nature of the original cell line and of several subclones. Measurement of the DNA content per metaphase showed a linear relation between chromosome number and DNA content of heteroploid cells. This finding is discussed with regard to its implications for the mechanism of heteroploidy in tumour cells.

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