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DNA amplification and an unstable arginine gene in Streptomyces lividans 66

✍ Scribed by Altenbuchner, Josef ;Cullum, John

Publisher: Springer
Year: 1984
Tongue: English
Weight: 472 KB
Volume: 195
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00332735

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✦ Synopsis

Streptomyces lividans 66 produced spontaneous chloramphenicol-sensitive mutants (CmlS) at a frequency of about 1% of spores. The CmlS mutant strains were very unstable, giving Arg- mutants at frequencies of about 25% of spores. All the Arg- mutants had amplified a particular 5.75 kb DNA fragment into tandem repeats of 250-500 copies per chromosome.

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