𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Divergent Genetic Control of Protein Solubility and Conformational Quality in Escherichia coli

✍ Scribed by Elena García-Fruitós; Mónica Martínez-Alonso; Nuria Gonzàlez-Montalbán; Minoska Valli; Diethard Mattanovich; Antonio Villaverde

Book ID
116663590
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
702 KB
Volume
374
Category
Article
ISSN
0022-2836

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Yield, solubility and conformational qua
Yield, solubility and conformational quality of soluble proteins are not simultaneously favored in recombinant Escherichia coli
✍ Mónica Martínez-Alonso; Elena García-Fruitós; Antonio Villaverde 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 235 KB

## Abstract Many enzymes or fluorescent proteins produced in __Escherichia coli__ are enzymatically active or fluorescent respectively when deposited as inclusion bodies. The occurrence of insoluble but functional protein species with native‐like secondary structure indicates that solubility and co

Prediction of protein solubility in Esch
Prediction of protein solubility in Escherichia coli using logistic regression
✍ Armando A. Diaz; Emanuele Tomba; Reese Lennarson; Rex Richard; Miguel J. Bagajew 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 254 KB 👁 1 views

## Abstract In this article we present a new and more accurate model for the prediction of the solubility of proteins overexpressed in the bacterium __Escherichia coli__. The model uses the statistical technique of logistic regression. To build this model, 32 parameters that could potentially corre

Recombinant protein expression and solub
Recombinant protein expression and solubility screening in Escherichia coli: a comparative study
✍ Berrow, Nick S. ;Büssow, K. ;Coutard, B. ;Diprose, J. ;Ekberg, M. ;Folkers, G. E 📂 Article 📅 2006 🏛 International Union of Crystallography 🌐 English ⚖ 522 KB

Producing soluble proteins in __Escherichia coli__ is still a major bottleneck for structural proteomics. Therefore, screening for soluble expression on a small scale is an attractive way of identifying constructs that are likely to be amenable to structural analysis. A variety of expression-screeni

Observations of green fluorescent protei
Observations of green fluorescent protein as a fusion partner in genetically engineered Escherichia coli: Monitoring protein expression and solubility
✍ Hyung Joon Cha; Chi-Fang Wu; James J. Valdes; Govind Rao; William E. Bentley 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 442 KB 👁 2 views

We have constructed three plasmid vectors for the expression of green fluorescent protein (GFP) fusion proteins using the following motif: (His) 6 -GFP-EK-X, where X represents chloramphenicol acetyl-transferase (CAT), human interleukin-2 (hIL-2), and organophosphorous hydrolase (OPH), respectively,