𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Screening of genetic parameters for soluble protein expression in Escherichia coli

✍ Scribed by Erik Vernet; Alexander Kotzsch; Bjørn Voldborg; Michael Sundström

Book ID
116829261
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
655 KB
Volume
77
Category
Article
ISSN
1046-5928

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Recombinant protein expression and solub
Recombinant protein expression and solubility screening in Escherichia coli: a comparative study
✍ Berrow, Nick S. ;Büssow, K. ;Coutard, B. ;Diprose, J. ;Ekberg, M. ;Folkers, G. E 📂 Article 📅 2006 🏛 International Union of Crystallography 🌐 English ⚖ 522 KB

Producing soluble proteins in __Escherichia coli__ is still a major bottleneck for structural proteomics. Therefore, screening for soluble expression on a small scale is an attractive way of identifying constructs that are likely to be amenable to structural analysis. A variety of expression-screeni

Combinatorial library approaches for imp
Combinatorial library approaches for improving soluble protein expression in Escherichia coli
✍ Hart, Darren J. ;Tarendeau, Franck 📂 Article 📅 2005 🏛 International Union of Crystallography 🌐 English ⚖ 299 KB

High-throughput screening methodologies are already used in structural biology to define efficient protein crystallization and expression conditions. Recently, screening approaches have been extended to the optimization of genetic constructs for improved soluble protein expression. With similarities