## Abstract A whole cell lysate of human cells was separated into 80 fractions according to the p__I__ of proteins using free flow isoelectric focusing with carrier ampholytes. The resolution of the process was highly reproducible, with an overlap of fractions of less than 30%. A protein of a faint
✦ LIBER ✦
Divergent flow isoelectric focusing: fast and efficient method for protein sample preparation for mass spectrometry
✍ Scribed by Karel Mazanec; Janette Bobalova; Karel Šlais
- Book ID
- 105892178
- Publisher
- Springer
- Year
- 2009
- Tongue
- English
- Weight
- 354 KB
- Volume
- 393
- Category
- Article
- ISSN
- 1618-2650
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Identification and detailed characterization of complex mixtures of proteins separated by polyacrylamide gel electrophoresis (PAGE) require optimized and robust methods for interfacing electrophoretic techniques to mass spectrometry. Peptide mapping by matrix-assisted laser desorption/ionization-tim