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Disturbance of hepatocyte morphology and function and of the hepatic microcirculation during long-term extracorporeal circulation in dogs

✍ Scribed by V. S. Sergeeva; A. I. Novokshonov; L. K. Altukhova; T. F. Chkhikvadze


Publisher
Springer US
Year
1981
Tongue
English
Weight
896 KB
Volume
91
Category
Article
ISSN
0007-4888

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A serum-free culture system for primary hepatocytes which maintains stable high-level hepatocyte function for prolonged periods in culture has been developed. Isolated rat primary hepatocytes were cultured in serum-free media between two layers of gelled collagen in a sandwich configuration which re