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Distribution of a major surface-associated glycoprotein, fibronectin, in cultures of adherent cells

✍ Scribed by Mosher, Deane F. ;Saksela, Olli ;Keski-Oja, Jorma ;Vaheri, Antti

Publisher
Wiley (John Wiley & Sons)
Year
1977
Tongue
English
Weight
468 KB
Volume
6
Category
Article
ISSN
0091-7419

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✦ Synopsis

Abstract

Fibronectin was present in media and cell layers of cultures of adherent cells from human skin, kidney, lung, chest wall, liver, and heart. Cell‐surface fibronectin, visualized by immunofluorescence, was in dense fibrillar (cultures from lung), discrete fibrillar (e.g., cultures from skin), or punctate (some cultures from kidney) structures. The subunit sizes of cell‐surface fibronectin and fibronectin soluble in medium appeared identical in sodium dodecyl sulfate‐polyacrylamide gels. To explain the polymorphism of cell‐surface fibronectin, there must be chemical differences among the fibronectins synthesized by different cell strains or factors in the cell layer which influence fibronectin binding and aggregation.

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