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Glycoprotein synthesis in the mucous cells of the vascularly perfused rat stomach. I. Surface mucous cells

✍ Scribed by Van Huis, G. A. ;Kramer, M. F.

Publisher
Wiley (John Wiley & Sons)
Year
1979
Tongue
English
Weight
997 KB
Volume
156
Category
Article
ISSN
0002-9106

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✦ Synopsis

Abstract

We developed a constant‐pressure vascular perfusion system of the isolated rat stomach, utilizing an artificial, fluorocarbon (FC‐75)‐containing medium. Perfusion could be maintained for at least six hours, as demonstrated by the ultrastructure of the mucosal cells and by the constant incorporation of [^3^H]‐galactose in the surface mucous cells. Moreover all mucous cell types in tissue fixed after six hours of perfusion showed the same histochemical reactions for glycoproteins as in tissue fixed shortly after decapitation of the animal.

The surface mucous cells of the antrum incorporated 30% less [^3^H]‐galactose, [^3^H]‐serine and [^35^S]‐sulphate than those of the fundus. The amount of radioactivity incorporated per cell did not decrease during a subsequent 2 hour chase.

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