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Distribution of 5-doxylstearic acid in the membranes of mammalian cells

โœ Scribed by D.O. Nettleton; P.D. Morse II; J.W. Dobrucki; H.M. Swartz; N.J.F. Dodd

Book ID
115728662
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
421 KB
Volume
944
Category
Article
ISSN
0005-2736

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The rate at which stearic acid nitroxide spin labels distribute through cells affects the interpretation of data obtained from these nitroxides. We used photoreduction of 5-doxylstearic acid (5DS) to determine the rate at which 5DS arrives at the outer half of the plasma membrane of mouse lymphocyte

Synthesis of 15N-5-doxylstearic acid for
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15N-5-Doxylstearic acid has been synthesized to improve EPR spectral characterization of the motional properties of the lipid in biological membranes. The EPR spectrum of the title compound displayed a 1.73 fold gain in sensitivity compared with the corresponding label substituted with the common i