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Distinguishing isobaric amino acids in sequence analysis of cyclosporins by fast atom bombardment and linked-scan mass spectrometry

✍ Scribed by Vladimír Havlíček; Alexandr Jegorov; Petr Sedmera; Winfried Wagner-Redeker; Miroslav Ryska

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
728 KB
Volume
30
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

An analytical protocol suggested previously for the mass spectrometric sequence analysis of cyclosporins was extended by addition of methods for discrimination among isobaric amino acids differing either in the nature of the side‐chain or N‐substitution. The former goal was achieved by comparison of B/E linked‐scan mass spectra of corresponding collisionally activated immonium ions with those of standard amino acids and the latter by comparison of B/E fragment ion spectra of [M + H]^+^ and [M~d~ + ^2^H]^+^ ions. The use of the improved protocol is illustrated by the structure elucidation of a novel natural cyclosporin, [Leu^9^]CS.

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