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Distance-dependent fluorescence quenching observed by frequency-domain fluorometry

✍ Scribed by Joseph R. Lakowicz; Józef Kuśba; Henryk Szmacinski; Michael L. Johnson; Ignacy Gryczynski

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
732 KB
Volume
206
Category
Article
ISSN
0009-2614

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✦ Synopsis

We describe frequency-domain measurements of the time-dependent intensity decays of pbis [2-(5-phenyloxazolyl) Ibenzene (PCJPOP) when collisionally quenched by carbon tetrabromide ( CBr,). Tbe frequency-domain data reveal the existence of a quenching rate constant which depends exponentially on the fluorophore-quencher separation. A distance-dependent quenching rate explains the decadesold observation of upward curvature in Stem-Volmer plots.

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