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Dissecting the fertilization process with monoclonal antibodies against sperm

โœ Scribed by AC Menge; DE Richter; RK Naz; C-YG Lee; E Wong

Book ID
119090165
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
116 KB
Volume
5
Category
Article
ISSN
0165-0378

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Hybridoma cell lines were produced by PEG (polyethylene glycol)-induced fusion of myeloma cells (NS-1/X63 Ag 8) with dispersed spleen cells from a Balb/c male mouse immunized syngeneically with homogenized Balb/c testis. Indirect immunofluorescence on mature (cauda epididymal) mouse sperm was employ