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Isolation of a monoclonal antibody against the acrosomal region of human sperm

✍ Scribed by V Garcia-Framis; W.A Lepp; P Martinez; J.M Benet-Rubinat; P Andolz; M.A Bielsa; J Egozcue

Book ID
119090755
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
56 KB
Volume
15
Category
Article
ISSN
0165-0378

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## Abstract A monoclonal antibody (MAb) against human acrosome‐reacted sperm was attached to paramagnetic polystyrene beads. Human sperm prepared by the swim‐up method were (1) incubated in m‐BWW, (2) incubated and ionophore treated, or (3) incubated with 5% seminal fluid. After treatment, sperm we