A tr/s-boronic acid receptor selective for glucose-6-phosphate is employed along with 5carboxyfluorescein as an indicator in a competitive spectrophotometric assay to determine glucose-6-phosphate concentrations in the mioromolar range.
โฆ LIBER โฆ
Discrimination between glucose-1-phosphate and glucose-6-phosphate with a boronic-acid-appended metalloporphyrin
โ Scribed by Tomoyuki Imada; Hideomi Kijima; Masayuki Takeuchi; Seiji Shinkai
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- French
- Weight
- 183 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0040-4039
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Two glucose-6-phosphate dehydrogenase (G6PD) variants were investigated. G6PD Amman-1 was partially purified from the red cells of a patient suffering from recurrent jaundice and spontaneous episodic attacks of severe hemolysis in the absence of oxidant drugs, infection, or fava beans. The enzymatic