Rational design of an improved induction
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Diethard Mattanovich; Walter Kramer; Christine Lüttich; Robert Weik; Karl Bayer;
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Article
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1998
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John Wiley and Sons
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English
⚖ 48 KB
👁 2 views
In Escherichia coli, strong overexpression of a recombinant protein has been shown to be deleterious due to a heavy metabolic burden on the host cell, which may completely cease cell growth before maximum product accumulation has occurred. Aiming at a reduction of very high product formation rates,