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Discovering the sweeping power of point mutations using a GIRAFF

โœ Scribed by Evgeni V Sokurenko

Book ID
117679952
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
55 KB
Volume
9
Category
Article
ISSN
0966-842X

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The molecular genetics of haemophilia A:
The molecular genetics of haemophilia A: screening for point mutations in the factor VIII gene using the restriction enzymeTaqI
โœ David S. Millar; Barbara Zoll; Uri Martinowitz; Vijay V. Kakkar; David N. Cooper ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Springer ๐ŸŒ English โš– 758 KB

A combination of Southern blotting and the analysis of polymerase chain reaction (PCR) amplified DNA fragments was used to screen the factor VIII genes of 527 haemophilia A patients for point mutations within TaqI restriction sites. Since this "directed search" strategy yielded only four gene lesion