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Directional cloning of native PCR products with preformed sticky ends (Autosticky PCR)

✍ Scribed by Gál, J. ;Schnell, R. ;Szekeres, S. ;Kálmán, M.


Book ID
106133191
Publisher
Springer
Year
1999
Tongue
English
Weight
96 KB
Volume
260
Category
Article
ISSN
0026-8925

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## Abstract In order to terminate the polymerase reaction at a desired position, a caged thymine derivative—4‐__O__‐[2‐(2‐nitrophenyl)propyl]thymine—was incorporated into PCR primers. In the PCR cycles, the elongation of the nascent strand (5′→3′ direction) by polymerase was site‐selectively termin